Fast ELISA kit for the quantitative determination of deoxynivalenol in cereals and beer.

Overall process times were geared to requirements as defined by the USDA GIPSA test performance specifications.

Method: Competitive enzyme immunoassay

Detection limit: 80 ppb
Wheat (110 ppb) - Rye (150 ppb) - Barley (110 ppb) - Aoin (150 ppb) - Corn (160 ppb) - Rice (150 ppb) - Beer (40 ppb)

Quantification limit: 200 ppb

Assay time: 20 min

Detection: 450 nm

Recovery rate:
Wheat (97%) - Rye (nd) - Barley (nd) - Oats (98%) - Corn (102%) - Rice (107%) - Beer (104%)

Standards: 0; 200; 500; 1 000; 2 000; 5 000 [ppb]

Ref. Range: 0 - 5 000 ppb

Cross reaction:
- 15-Acetyl-DON 0.4%
- 3-Acetyl-DON 800%


Storage temperature: 2 - 8ºC

Format: Microplate - 96 wells

- Quantitative results based on superior 4PL curve fitting for calibration
- Microsoft Excel-based calculation & evaluation tool available
- Reagents (excl. wash buffer) ready-to-use
- 96 well format with breakable microwell strips
- Simple extraction procedures
- Incubation at room temperature
- High accuracy and reproducibility
- High stability; minimum shelf life 12 months

Cereals and beer